goat anti wnt2 antibody  (R&D Systems)

Journal: Genes & Cancer

Article Title: Biological significance and therapeutic implication of resveratrol-inhibited Wnt, Notch and STAT3 signaling in cervical cancer cells

doi:

Figure Lengend Snippet: A: Immunocytochemical illustration of intracellular distribution of Notch1, Notch2, Hes1, Wnt2, Wnt5a, β-catenin, p-STAT3 and PIAS3 in HeLa and SiHa cells without (N) and with (R) 100 μM resveratrol treatment for 48h. Immunofluorescent staining results were showed on the up-left corners. B: HE staining in HeLa and SiHa cells without (N) and with (R) 100 μM resveratrol treatment for 48h. C: Trypan blue discrimination of stained (unviable) and unstained (viable) cells. HeLa and SiHa cells were treated with 100 μM resveratrol for 0h, 12h, 24h, 36h and 48h respectively.

Article Snippet: The antibodies used were a mouse anti-human Notch1 monoclonal antibody (1:100, NeoMarkers, Inc, Fremont, California), a rabbit anti-human Notch2 polyclonal antibody (1:100, Santa Cruz, CA, USA), a rabbit anti-human Hes1 polyclonal antibody (1:1000; a generous gift of Tetsuo Sudo, PhD, Toray Industries, Tokyo, Japan), a goat anti-human Wnt2 (1:100; Santa Cruz, CA, USA), a rabbit anti-human Wnt5a polyclonal antibody(1:100; Santa Cruz, CA, USA), a mouse anti-human β-catenin (1:100; Santa Cruz, CA, USA), a rabbit anti-human p-STAT3(1:100; Santa Cruz, CA, USA) and a rabbit anti-human PIAS3 polyclonal antibody (1:100; Santa Cruz, CA, USA).

Techniques: Staining

Journal: Genes & Cancer

Article Title: Biological significance and therapeutic implication of resveratrol-inhibited Wnt, Notch and STAT3 signaling in cervical cancer cells

doi:

Figure Lengend Snippet: A: Evaluation of Notch1, Notch2, Hes1, Wnt2, Wnt5a, β-catenin, p-STAT3 (STAT3) and PIAS3 in HeLa and SiHa cells without (N) and with (R) 100 μM resveratrol treatment for 48h by Western blotting and B: RT-PCR, β-actin was used as a quantitative control. Gray analyses of the Western blotting and RT-PCR results were under each picture, the ordinate was the ratio of each index and β-actin grey value.

Article Snippet: The antibodies used were a mouse anti-human Notch1 monoclonal antibody (1:100, NeoMarkers, Inc, Fremont, California), a rabbit anti-human Notch2 polyclonal antibody (1:100, Santa Cruz, CA, USA), a rabbit anti-human Hes1 polyclonal antibody (1:1000; a generous gift of Tetsuo Sudo, PhD, Toray Industries, Tokyo, Japan), a goat anti-human Wnt2 (1:100; Santa Cruz, CA, USA), a rabbit anti-human Wnt5a polyclonal antibody(1:100; Santa Cruz, CA, USA), a mouse anti-human β-catenin (1:100; Santa Cruz, CA, USA), a rabbit anti-human p-STAT3(1:100; Santa Cruz, CA, USA) and a rabbit anti-human PIAS3 polyclonal antibody (1:100; Santa Cruz, CA, USA).

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Genes & Cancer

Article Title: Biological significance and therapeutic implication of resveratrol-inhibited Wnt, Notch and STAT3 signaling in cervical cancer cells

doi:

Figure Lengend Snippet: Immunohistochemical profiling of Notch1, Notch2, Hes1, Wnt2, Wnt5a, β-catenin, p-STAT3 and PIAS3 expression in human cervical tissues (N: normal cervical tissues removed from the uterine fibroidpatients at post-reproduction ages, AC: cervical adenocarcinomas, SC: cervical squamous cell carcinomas. * p <0.01)

Article Snippet: The antibodies used were a mouse anti-human Notch1 monoclonal antibody (1:100, NeoMarkers, Inc, Fremont, California), a rabbit anti-human Notch2 polyclonal antibody (1:100, Santa Cruz, CA, USA), a rabbit anti-human Hes1 polyclonal antibody (1:1000; a generous gift of Tetsuo Sudo, PhD, Toray Industries, Tokyo, Japan), a goat anti-human Wnt2 (1:100; Santa Cruz, CA, USA), a rabbit anti-human Wnt5a polyclonal antibody(1:100; Santa Cruz, CA, USA), a mouse anti-human β-catenin (1:100; Santa Cruz, CA, USA), a rabbit anti-human p-STAT3(1:100; Santa Cruz, CA, USA) and a rabbit anti-human PIAS3 polyclonal antibody (1:100; Santa Cruz, CA, USA).

Techniques: Immunohistochemical staining, Expressing

Journal: Genes & Cancer

Article Title: Biological significance and therapeutic implication of resveratrol-inhibited Wnt, Notch and STAT3 signaling in cervical cancer cells

doi:

Figure Lengend Snippet: A: Tissue microarray-based immunohistochemical staining for Notch1, Notch2, Hes1, Wnt2, Wnt5a, β-catenin, p-STAT3 and PIAS3 in normal cervical tissues removed from the uterine fibroid patients at post-reproduction ages (N), cervical adenocarcinomas (AC) and cervical squamous cell carcinomas (SC) (original magnifications×400). B: Histogram of Table , *p<0.01.

Article Snippet: The antibodies used were a mouse anti-human Notch1 monoclonal antibody (1:100, NeoMarkers, Inc, Fremont, California), a rabbit anti-human Notch2 polyclonal antibody (1:100, Santa Cruz, CA, USA), a rabbit anti-human Hes1 polyclonal antibody (1:1000; a generous gift of Tetsuo Sudo, PhD, Toray Industries, Tokyo, Japan), a goat anti-human Wnt2 (1:100; Santa Cruz, CA, USA), a rabbit anti-human Wnt5a polyclonal antibody(1:100; Santa Cruz, CA, USA), a mouse anti-human β-catenin (1:100; Santa Cruz, CA, USA), a rabbit anti-human p-STAT3(1:100; Santa Cruz, CA, USA) and a rabbit anti-human PIAS3 polyclonal antibody (1:100; Santa Cruz, CA, USA).

Techniques: Microarray, Immunohistochemical staining, Staining

Journal: Genes & Cancer

Article Title: Biological significance and therapeutic implication of resveratrol-inhibited Wnt, Notch and STAT3 signaling in cervical cancer cells

doi:

Figure Lengend Snippet: The primer sequences of polymerase chain reactions

Article Snippet: The antibodies used were a mouse anti-human Notch1 monoclonal antibody (1:100, NeoMarkers, Inc, Fremont, California), a rabbit anti-human Notch2 polyclonal antibody (1:100, Santa Cruz, CA, USA), a rabbit anti-human Hes1 polyclonal antibody (1:1000; a generous gift of Tetsuo Sudo, PhD, Toray Industries, Tokyo, Japan), a goat anti-human Wnt2 (1:100; Santa Cruz, CA, USA), a rabbit anti-human Wnt5a polyclonal antibody(1:100; Santa Cruz, CA, USA), a mouse anti-human β-catenin (1:100; Santa Cruz, CA, USA), a rabbit anti-human p-STAT3(1:100; Santa Cruz, CA, USA) and a rabbit anti-human PIAS3 polyclonal antibody (1:100; Santa Cruz, CA, USA).

Techniques: Sequencing, Countercurrent Chromatography